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AIM:To study the chemical constituents of the roots of Astragalus hoantchy Franch.. METHOD:Isolation and elucidation of the chemical constituents,were conducted by chromatography and spectral evidences. RESULTS and CONCLUSION:Six steroids and four anthraquinones were isolated from the roots of A. hoantchy. Their structures were identified to be stigmastane-3,6-dione (1),5α,8α-epidioxy- (22E,24R)-ergosta-6,22-dien-3β-ol (2),stigmastane-3β,6α-diol (3),daucosterol (4),β-sitosterol (5),stigmasterol (6),chrysophanol (7),emodin (8),physion (9) and aloe-emodin (10) on the basis of spectral data and physical constants. Among them,compounds 1,2,3,7,8,9,10 were isolated from the genus Astragalus for the first time.
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AIM To investigate the chemical constituents from the rhizomes of Cimicifuga acerina (Sieb. et Zucc.) Tanaka. METHODS Column chromatgraphy (including silica gel and ODS) together with HPLC was used to separate the chemical constituents whose structures were determined by FAB-MS, NMR (1D and 2D) and hydrolysis methods. RESULTS Five cycloartane triterpenoids were isolated and identified as: (22R)-22-hydroxycimigenol (I), (22R)-22-hydroxy-24-O-acetylhydroshengmanol 3-O-β-D-xylopyranoside (II), dahurinol (III), 24-epi-24-O-acetyl-7,8-didehydroshengmanol 3-O-β-D-xylopyranoside (IV), 25-O-acetyl-7,8-didehydrocimigenol 3-O-β-D-xylopyranoside (V). CONCLUSION Compound I is a new natural product, compound II is a new compound and compounds IV and V were isolated from this plant for the first time.
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Objective To study the variance of fingerprint peaks in the processes of Anti-aging Compound Recipe formation,compatibility,and preparation.Methods Anti-aging Compound Recipe and its herbs′ fingerprint chromatogram were studied and validated by HPLC/UV and LC/MS which were used in chemical compatibility.Results Based on the 18 code peaks from compound recipe fingerprint and compared with herb fingerprints,18 code peaks of compound recipe were under the assignment of herb source,that was,No.1,2 from Ganoderma No.11L,21L;No.4,5 from Fructus Ligustri Lucidi No.1N,4N;No.3,8,9,10,11,12,13,14, 18 from Fructus Schisandrae.No.1W,4W,12W,14W,16W,18W,22W,26W,31W;and No.6,7,15,16,17 were no assignment.The assignment resulted the peaks of herb source could be divided into several fingerprint regions,as anti-aging formulae,6—14 min mainly from Ganoderma, 20—30 min from Fructus Ligustri Lucidi,after 40 min from Fructus Schisandrde.All of them enhanced what the study on chemical compatibility of Anti-aging Compound Recipe was aimed at.Conclusion The two actions have appeared during mixing the crude Chinese medicinal materials into a formula recipe,one is no influence among the coexisted raw materials,while the other is with influence each other.In Anti-aging Compound Recipe the three co-decocted raw materials and their sequence put into are considered.Final results present that Fructus Schisandrae adding sequence could influence the quality of compound recipe which illustrated the compatibility among herbs and determined the variance of herbs during the preparation of compound recipe.And the results also provide a feasible way to study the compatibility and quality of compound recipe.
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AIM: Provided the integrated method for large TCM recipe chemical study with the establishment of Aozhou formulae,imcluding 20 herbs. METHODS: With the comparison of Aozhou formulae and its herbs fingerprint chromatogram,it extracted fingerprint characters from chromatogram after the method establishment.According to the test results,the retention data,on-line UV and MS spectral were collected as the Aozhou formulae fingerprint database.Combined with three character classified data layers named as herb jurisdiction,multi herb jurisdiction and no herb jurisdiction,the Aozhou formulae was identified by database mentioned above with the 10 batches results. RESULTS: It presented that formulae identification by character data was more accurate than by LC/UV or LC/MS data merely.According to the comparison with median, the deviation were ?2.7%,?10.5%,(?11.2%) identified by character, LC/UV and LC/MS respectively. CONCLUSION: From the method described in this article,it could be more accurate and sensitive to reflect quality and substance characteristic by the comparison of characters extracted from compound recipe fingerprint chromatogram.And also it provided method to study the substance basement of large TCM formulae.